“That Really Rustled My Jimmies” is an expression that is used to convey feelings of discontent or discomfort in response to someone else’s post in imageboards and discussion forums, most notably on 4chan. To rustle one's jimmies means to make someone discontent or to bother someone.
Man 2: "That really does Rustle My Jimmies man..."
By Karrie
Rustling My Jimmies
Verb: To erk someone. To be all up in their biz. To cause frustration and anxiety. Basically to piss someone off in a profound way. Or, to bother someone's friends named jimmy
You: yo mama...
Me: I can't stand it when you rustle my jimmies like that
as others have stated: something has angered, upset, annoyed, aroused you
this is more of a side note on origin - I suspect the phrase was a spontaneous misquote of a line from the Weezer song "Say It Ain't So" ("...flip on the telly/ wrestle with Jimmy...")